近日，Nature发表了中科院微生物所关于新冠中和抗体的重大研究成果，他们从康复患者筛选出了两株全人源单克隆抗体CB6和CA1， 其中CB6克隆在非人灵长类动物试验模型中能够有效治疗和预防新冠病毒感染。目前国家药品监督管理局已经正式批准CB6（JS016）的临床试验申请。CR3022单抗克隆株是一株从非典肺炎康复患者体内分离出的全人源化单克隆抗体。这株抗体虽然可以和新冠病毒交叉反应，但是不具备中和和抑制病毒感染的功能，是一株作为广泛研究的参照抗体。
Figure 1. CB6 can block SARS-CoV2-RBD binding to hACE2 in FACS-based assay
(图片来源：Nature)
目前检测新冠病毒的主要方法是核酸检测和免疫学检测，采取核酸检测为主，免疫学检测为辅的策略。其中免疫学检测分为抗原检测和抗体检测两种，对于免疫学检测来说，优质的抗原抗体对于检测的灵敏度和特异性至关重要。缔码生物应用HEK293人源细胞表达平台制备的重组新冠病毒S蛋白更接近于天然翻译后修饰，并且我们也对表达纯化的S蛋白测试进行了ACE2受体蛋白结合功能验证和多种已发表新冠病毒中和性单克隆抗体结合功能验证（包括最新的CB6和CR3022单抗克隆）。
CB6单克隆抗体可以有效的抑制新冠病毒S-RBD蛋白结合ACE2受体蛋白
Figure 2. ELISA plate coated with recombinant SARA-CoV-2 S protein RBD was first pre-blocked with recombinant CB6 antibody or recombinant CR3022 antibody, and then 100 ng/ml Human ACE2, mFc tagged protein ([getskuurl sku=”PME100072″]) was added to check the competitive inhibition of recombinant CB6 antibody and recombinant CR3022 antibody. This data indicated that recombinant CB6 antibody can efficiently block the binding between SARS-CoV-2 S protein RBD domain with Human ACE2 protein.
好礼赠送
为了方便客户进行蛋白功能验证和应用CB6和CR3022作为参照抗体进行研发，我们所有销售的新冠蛋白产品都会附送重组表达的CB6和CR3022单克隆抗体。
产品推荐
SARS-CoV-2 (2019-nCoV) S protein RBD, mFc-His Tag
Catalog Number: [getskuurl sku=”PME100460″]
Expression Host: HEK293
Tag: C-Mouse Fc and 6×His tag
Purity: Greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Figure 3. SARS-CoV-2 (2019-nCoV) S protein RBD, mFc-His Tag on SDS-PAGE under reducing condition.
Figure 4. ELISA plate pre-coated by 2 μg/ml (100 μl/well) SARS-CoV-2 (2019-nCoV) S protein RBD, mFc-His tagged protein ([getskuurl sku=”PME100460″]) can bind Anti-SARS-CoV-2 S protein RBD Neutralizing Antibody (A neutralizing monoclonal antibody clone currently under clinical investigation from collaboration company) in a linear range of 0.24-9.141 ng/ml.
Human ACE2 Protein, mFc Tag
Catalog Number: [getskuurl sku=”PME100072″]
Expression Host: HEK293
Tag: C-Mouse Fc tag
Purity: Greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Figure5.Human ACE2 Protein, mFc Tag on SDS-PAGE under reducing condition.
SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein, His Tag
Catalog Number: [getskuurl sku=”PME100459″]
Expression Host: E.coli
Tag: C-His tag
Purity: Greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Figure 6. SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein, His Tag on SDS-PAGE under reducing condition.除了重组蛋白产品的开发，我们的缔码重组单克隆抗体制备平台也正在应用于新冠病毒相关重组单克隆抗体开发。与传统的杂交瘤制备平台相比，我们拥有更高的筛选效率，从单只免疫动物中我们能够筛选到上千甚至上万个阳性B细胞克隆，这些阳性克隆能够帮助我们筛选拥有更多表位和适用于更多免疫学应用的抗体。我们的新冠抗体开发正在进行中，欢迎广大客户关注我们，来电咨询更多相关信息。
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